Bio-rad伯樂EveryBlot Blocking Buffer封閉液 12010020
Bio-rad伯樂EveryBlot Blocking Buffer封閉液 12010020 | 時間: May-6 15:49:36 | 分類:其它儀器
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產(chǎn)品描述
一瓶封閉緩沖液，500毫升。所有western印跡只需要5分鐘的封閉
每個印跡-5分鐘每個印跡阻斷
五分鐘的阻斷緩沖液，適用于所有印跡。
EveryBlot阻斷緩沖液為所有蛋白質(zhì)印跡提供5分鐘的阻斷和較大靈敏度，無論檢測方法如何。
Bio-rad伯樂EveryBlot Blocking Buffer封閉液 12010020
功能和優(yōu)點
減少非特異性抗體結(jié)合以減少背景，同時保持優(yōu)異的敏感性
用于化學(xué)發(fā)光和熒光檢測
不含磷酸鹽緩沖液。非常適合與磷酸特異性抗體一起使用
5分鐘內(nèi)完成封閉
Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots
competitor-everyblot-5 minute everyblot blocking
A five minute blocking buffer for ALL western blots.
EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.
Features and Benefits
Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity
For both chemiluminescent and fluorescent detection
Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies
Complete blocking in 5 minutes
Bio-rad伯樂EveryBlot Blocking Buffer封閉液 12010020
使用建議
對于小型印跡，使用至少10 ml用于阻斷和抗體孵育步驟。對于中等大小的印跡，至少使用20毫升。
將塊加入膜中，攪拌孵育5分鐘
將第一抗體和第二抗體稀釋在全強(qiáng)度塊中，并在攪拌下孵育1小時
對于PVDF的熒光檢測，將SDS添加到第二抗體溶液中的0.02%。
Recommended Protocol
For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.
Add block to the membrane and incubate for 5 minutes with agitation
Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation
For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.
